Recombinant bovine porcine IGF-1LR3

Details

Catalog number: CM0013

Format: 50ug, 100 ug, 500 ug, 1.000ug

Origin: It is produced by transient expression in non-transgenic plants and purified by sequential chromatography steps. This product contains no animal–derived components or impurities.

Description: Insulin-like growth factor I (IGF-I) belongs to the insulin-like growth factor family of signaling molecules that play critical roles in cellular energy metabolism and in growth and development. IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. IGF-1 shares a high degree of structural and functional homology with the insulin hormone. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF I induces endothelial cell migration and is involved in the regulation of angiogenesis. It is a mitogenic agent for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. It also plays an important role in some differentiation and anti-apoptotic functions, so it has a key role in tissue renewal and repair. In tissues, IGF-I is produced by mesenchymal type cells and acts in a paracrine fashion and /or an autocrine fashion by binding to the IGF-IR. This binding activates the receptor tyrosine kinase that triggers the downstream responses and finally stimulates cell division. His potent mitogenic activity is determinate by the availability of free IGF-1 to interact with IGF-1 receptors. Insulin and the IGFs elicit the same biological responses, however, a number of studies have shown that the receptor IGF1-R, is highly expressed on muscle stem cells compared to the Insulin receptor and results have furthermore demonstrated that IGF-1 is a more potent mitogenic regulator of primary myoblast and myotubes than Insulin.IGF-1 LR3 retains the pharmacological activity of IGF-1 as an IGF-1 receptor agonist, has a very low affinity for insulin-like growth factor binding proteins (IGFBP), and has improved metabolic stability. It is about three times more potent than IGF-1 and possesses a significantly longer half-life of about 20-30 hours (relative to IGF-1's half-life of about 12-15 hours)

Species: Porcine/Bovine

Purity: > 95% by SDS-PAGE gel

Endotoxin: < 0.05 EU / μg protein (LAL method)

Formulation: Lyophilized from a Tris HCl buffer , NaCl and Urea, at pH 7.4. Sterile filtered through 0.2 µm filter.

Reconstitution: Lyophilized protein should be reconstituted in water following instructions of Certificate of Analysis (CoA). At higher concentrations solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and /or assay

Storage and Stability: The lyophilized product is stable between 2-8ºC. Once reconstituted, use immediately or store in aliquots at -20ºC (maximum shelf life 3 months). Avoid thawing and freezing.