Recombinant Bovine Fibroblast Growth Factor 21

Details

Catalog number: CM0025

Format: 50ug, 200ug, 500 ug

Origin: It is produced by transient expression in non-transgenic plants and purified by sequential chromatography steps. This product contains no animal–derived components or impurities.

Description: Fibroblast growth factor 21 is a member of endocrine FGFs subfamily, along with FGF19 and FGF23. It is emerging as a novel regulator with beneficial effects on a variety of metabolic parameters, including glucose and lipid control. Some studies reported that increasing of FGF21 could ameliorate obesity and diabetes through enhancing glucose uptake, suppressing adipogenesis, or increasing lipolysis both in vitro and in vivo. FGF21 activity depends on membrane protein beta Klotho that physically complexes with various FGF receptors, thus conferring them the ability to bind FGF21 and activate downstream signaling pathways. regulate cell growth, cell differentiation, embryonic development, metabolism, and trigger many other physiological and pathogenic processes. FGF21 also plays important roles in myogenesis. In the first few years, skeletal muscle was not considered as a source of FGF21. Later, one study carried out on mice demonstrated that skeletal muscle can also act as a source of FGF21 and it can be regulated via PI3K/Akt1 pathway. Moreover, some studies reported that FGF21 could be induced in skeletal muscle by hyperinsulinemia and exercise in human beings and is associated with myogenic differentiation and regulated by myogenic factor MyoD; also, FGF21 is involved in myofiber type transformation. FGF21 is currently considered as a myokine and adipokine, which mediates the cross talk between skeletal muscle and adipose tissue in energy metabolism.

Species: Bovine

Purity: > 95% by SDS-PAGE gel

Endotoxin: < 0.05 EU / μg protein (LAL method)

Formulation: Formulated from Phosphate Potassium buffer pH 7.5 and NaCl. Sterile filtered through a 0.22µm filter.

Reconstitution: Lyophilized protein should be reconstituted in water following instructions of Certificate of Analysis (CoA). At higher concentrations solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and /or assay

Storage and Stability: The lyophilized product is stable between 2-8ºC. Once reconstituted, use immediately or store in aliquots at -20ºC (maximum shelf life 3 months). Avoid thawing and freezing.