Molecular weight: Recombinant human IGF-1 is a polypeptide chain containing 70 amino acids (49 – 118 P05019-1 IGF1_HUMAN) with a His tag at N-terminal.
Description: Insulin-like growth factor I (IGF-I) belongs to the insulin-like growth factor family of signaling molecules that play critical roles in cellular energy metabolism and in growth and development. IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. IGF-1 shares a high degree of structural and functional homology with the insulin hormone.
IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis. It is a mitogenic agent for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. It also plays an important role in some differentiation and anti-apoptotic functions, so it has a key role in tissue renewal and repair.
In tissues, IGF-I is produced by mesenchymal type cells and acts in a paracrine fashion and /or an autocrine fashion by binding to the IGF-IR. This binding activates the receptor tyrosine kinase that triggers the downstream responses and finally stimulates cell division.
His potent mitogenic activity is determinate by the availability of free IGF-1 to interact with IGF-1 receptors. Insulin and the IGFs elicit the same biological responses, either by cross-reacting with one of the receptors for the heterologous ligand or by concurrent activation of convergent effector pathways by binding to the homologous receptor.
Figure 1. SDS-PAGE of recombinant IGF-1. Samples were loaded in 15% SDS-polyacrylamide gel and stained with coomassie blue. Lane MWM: Molecular weight marker (kDa); Lane 2: contains 100 ng of recombinant IGF-1.
Figure 2.- Western Blot analysis of recombinant IGF-1. Lane MWM: Molecular weight marker (kDa); Lane 2: contains 100 ng of recombinant IGF-1. .
1.The specific activity is determined by the dose-dependent proliferation of mouse BALBC 3T3 cells using a concentration range of 10-20 ng/ml.
Human recombinant protein expressed in Nicotiana benthamiana.
Bulk sizes are available upon request.
For R&D purposes only. Purchaser must determine the suitability of the product(s) for their particular use.